Haplogroup X2b4 is characterized by several distinctive mutations, as follows.

Haplogroup or Subgroup	Required Mutations
X	T6221C, C6371T, A13966G, T14470C, T16189C!, C16278T!
X2	T195C!, G1719A
X2b	C8393T, G15927A
X2b4	G3705A

Of the above mutations, only two, the mutations at 16189 and at 16278 are found in the HVR1 region, and only the mutation at 195 is found in the HVR2 region. The balance of these mutations are found in the coding region, so a haplogroup cannot be predicted at a higher level that X or perhaps X2 without the full sequence test.

Visit https://dna-explained.com/2016/09/14/haplogroup-x2b4-is-european-not-native-american/ for updated project information about the X2b4 mtDNA haplogroup.

When making inquiries about this project, please contact all project adminstrators (i.e., together, in a single email message) so that whomever might happen to be able to answer first, or whomever has the most appropriate expertise, can do so.